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KMID : 1004520220380030150
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Journal of Dental Rehabilitation and Applied Science
2022 Volume.38 No. 3 p.150 ~ p.161
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A study on the effect of microgroove-fibronectin complex titanium plate on the expression of various cell behavior-related genes in human gingival fibroblasts
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Hwang Yu-Jeong
Lee Won-Joong
Lee Sung-Bok Richard
Lee Suk-Won
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Abstract
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Purpose: To determine the effects of the microgroove-fibronectin complex surface on the expression of various genes related to cellularactivity in human gingival fibroblasts.
Materials and Methods: Smooth titanium specimens (NE0), acid-treated titanium specimens(E0), microgroove and acid-treated titanium specimens (E60/10), fibronectin-fixed smooth titanium specimens (NE0FN), acidtreatedand fibronectin-immobilized titanium specimens (E0FN), and microgroove and acid-treated titanium specimens immobilizedwith fibronectin (E60/10FN) were prepared. Real-time polymerase chain reaction experiments were conducted on 44 genes relatedto cell behavior of human gingival fibroblasts.
Results: Adhesion and proliferation of human gingival fibroblast on microgroovefibronectincomplex titanium were activated through four types of signaling pathway. Integrin α5, Integrin β1, Integrin β3, Talin-2,which belong to the focal adhesion pathway, AKT1, AKT2, NF-κB, which belong to the PI3K-AKT signaling pathway, MEK2, ERK1,ERK2, which belong to the MAPK signaling pathway, and Cyclin D1, CDK4, CDK6 genes belonging to the cell cycle signaling pathwaywere upregulated on the microgroove-fibronectin complex titanium surface (E60/10FN).
Conclusion: The microgroove-fibronectincomplex titanium surface can up-regulate various genes involved in cell behavior.
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KEYWORD
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titanium, fibronectin, gingival fibroblasts, cell behavior, gene expression
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